ru
ru
Source
Chromosome Research
DATE OF PUBLICATION
02/26/2023
Authors
Polina Belokopytova Veniamin Fishman Artem Nurislamov Maria Gridina Pavel Salnikov Anna Khabarova Galina Koksharova Roman Mungalov Inna Pristyazhnuk
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A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells

Cell line models, Chromosome rearrangement, Cre/LoxP system, Genome editing

Abstract

Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.

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